BRIEF REVIEW OF ELECTROMAGNETIC SPECTRUM AND ABSORPTION OF RADIATIONS:
Theory of spectroscopy, absorption by organic molecules, choice of solvent and solvent effects, modern instrumentation – design and working principle. Applications of UV-Visible spectroscopy (qualitative and quantitative analysis). Principles of vibrational spectroscopy, frequency and factors influencing vibrational frequency, instrumentation and sampling techniques, interpretation of spectra, applications in biology. FT-IRtheory and applications, Attenuated Total Reflectance (ATR). Raman Spectroscopy, theory, instrumentation, and applications to biology. Discussions with Case studies.
FUNDAMENTAL PRINCIPLES OF NMR:
Instrumentation, solvents, chemical shift, and factors affecting chemical shift, spin-spin coupling, coupling constant, and factors influencing the value of coupling constant, spin-spin decoupling, proton exchange reactions, FT-NMR, 2D -NMR, NMDR, NOE, NOESY, COSY and applications in Pharmacy, interpretation of spectra, C13 NMRIntroduction, Natural abundance, C13 NMR Spectra and its structural applications. Discussions with Case studies.
BASIC PRINCIPLES AND INSTRUMENTATION OF ION FORMATION AND TYPES:
Fragmentation processes and fragmentation pattern, Chemical ionization mass spectroscopy (CIMS), Field Ionization Mass Spectrometry (FIMS), Fast Atom Bombardment MS (FAB MS), Matrix Assisted laser desorption / ionization MS (MALDI-MS), GC-MS. LC-MS. MS-MS. Discussions with Case studies
INTRODUCTION TO X-RAY:
Generation of X-rays, Xray diffraction, Bragg’s law, X-ray powder diffraction, interpretation of diffraction patterns and applications. Single crystal diffractions of biolomolecules. Fibre diffraction. Neutron diffraction. XAFS. ORD Principle, Plain curves, curves with cotton effect, octant rule and its applications with example, circular dichroism and its relation to ORD.Discussions with Case studies
CHROMATOGRAFIC TECHNIQUES:
Classification of chromatographic methods based on mechanism of separation: paper chromatography, thin layer chromatography, ion exchange chromatography, column chromatography and affinity chromatography – technical questions and applications. Gas Chromatography: Theory and principle, column operation, instrumentation, derivatisation methods and applications. HPLC, LC-MS and applications in HPTLC. Discussions with Case studies.