INTRODUCTION AND METABOLIC REGULATION:
Introduction: Importance of metabolic engineering and its multidisciplinary nature. An overview of Cellular Metabolism, Transport Processes, Passive Transport, Facilitated Diffusion, Active Transport, Fueling Reactions, Fermentative Pathways, Glycolysis, TCA Cycle and Oxidative Phosphorylation, Anaplerotic Pathways, Catabolism of Fats, Organic Acids, and Amino Acids, Biosynthetic Reaction, Biosynthesis of Amino Acids, Biosynthesis of Nucleic Acids, Fatty Acids.
METABOLIC FLUX AND APPLICATIONS OF METABOLIC FLUX ANALYSIS:
Metabolic flux analysis and its application, Methods for experimental determination of metabolic flux by isotope dilution method. Production of Glutamic Acid and regulation by Bacteria, Calculation of Theoretical Yields, Metabolic Flux Analysis of Lysine Biosynthetic Network in C. glutamicum, Metabolic Flux Analysis of Specific Deletion Mutants of C. glutamicum, Metabolic Fluxes in Mammalian Cell Cultures, Determination of Intracellular Fluxes, Application of Flux Analysis to the Design of Cell Culture Media.
REGULATION OF METABOLIC PATHWAYS:
Regulation of Enzymatic Activity, Overview of Enzyme Kinetics, Simple Reversible Inhibition Systems, Irreversible Inhibition, Allosteric Enzymes: Cooperativity, Regulation of Enzyme Concentration, Control of Transcription Initiation, Control of Translation, Global Control: Regulation at the Whole Cell Level, Regulation of Metabolic Networks, Branch Point Classification, Coupled Reactions and the Role of Global Currency Metabolites.
METABOLIC ENGINEERING IN PRACTICE:
Enhancement of Product Yield and Productivity, Ethanol, Amino Acids, Solvents, Extension of Substrate Range, Metabolic Engineering of Pentose Metabolism for Ethanol Production, Cellulose-Hemicellulose Depolymerization, Lactose and Whey Utilization, Sucrose Utilization, Starch Degrading Microorganisms, Extension of Product Spectrum and Novel Products, Antibiotics, Polyketides, Vitamins, Biopolymers, Biological Pigments, Hydrogen, Pentoses: Xylitol, Improvement of Cellular Properties, Prevention of Overflow Metabolism, Alteration of Substrate Uptake, Maintenance of Genetic Stability.
BIOSYNTHESIS OF METABOLITES AND BIOCONVERSIONS:
Primary metabolites: Alteration of feedback regulation, limiting of accumulation of end products, resistant mutants. Secondary metabolites: Precursor effects, prophage, idiophase relationship, enzyme induction, feedback repression, catabolic repression, important groups of secondary metabolic enzymes, phosphotransferase, ligases oxido reductases, oxygenases, carboxylases.Advantages of bioconversions, specificity, yields. Factors important to bioconversions, regulation of enzyme synthesis, permeability co metabolism, conversion of insoluble substrates.