MTech Fermentation Technology syllabus for 2 Sem 2020 scheme 20BBC21

HISTORY OF DEVELOPMENT OF FERMENTATION INDUSTRY:

The range of fermentation process, Microbial biomass, enzymes, metabolites, recombinant products, Transformation process, the component parts of Fermenter. Types of industrial bioprocesses; submerged, surface, solid state fermentations: aerobic, anaerobic and light based processes. The differences between laboratory, pilot, and manufacturing scale bioreactor experiments, Green biologics of fermentation technology, types of Reactor and reactor design, process economics. Discussions with case studies

SCREENING OF IMPORTANT METABOLITES FROM MICROBIAL SOURCES:

Primary and secondary screening of industrially important microbes, Screening methods, General Techniques in improvement of industrial strains, Isolation of auxotrophic mutants, resistant mutants, revertant mutants, Selection by induced mutants producing improved yields of secondary metabolites. Preservation and storage at reduced temperature; Agar slopes, liquid nitrogen, dehydrated form, dried culture and lyophilisation. Quality control of reservation of stock cultures

INTRODUCTION TO CULTURE MEDIUM AND FORMULATION:

Energy sources, Carbon & Nitrogen sources, Minerals, Growth factors, Buffers, Precursors and regulators, Oxygen and antifoam ingredients, Medium optimization. Substrates for solid state fermentation, Evaluation methods for complex Substrates differences based on product use.

STERILIZATION PROCESS AND INOCULUM DEVELOPMENT

Medium sterilization, Design for Batch sterilization process, Calculation of del factors and holding time. Design of continuous sterilization process, Sterilization of Fermenters, Feeds & liquid wastes, Filter sterilization of media. Discussions with case studies Development of Inoculum, criteria for transfer, development of inoculum in yeast, bacterial and mycelial processes, aseptic inoculation of plant fermenters. Inoculum development methods.

LABORATORY TO LARGE SCALE FERMENTATION PROCESSES:

Batch, Continuous culture, Synchronous, nonsynchronous growth kinetics, Feedback systems, comparison of Batch and Continuous culture in industrial processes and investigative tools. Fed batch culture, Applications of Fed back cultures Techniques and trends in Fermentation technology for the production of recombinant vaccines, therapeutic proteins, antibiotics and diagnostics. Discussions with case studies. Treatment and disposal procedure for industrial effluents.

Course outcomes:

After studying this course, students will be able to:

• 
  Demonstrate strong basics in principles of fermentation technology
  
• Demonstrate strong basics numerical analysis,
  
• Design and develop various fermentation processes
  

Question paper pattern:

The SEE question paper will be set for 100 marks and the marks scored will be proportionately reduced to 60.

• The question paper will have ten full questions carrying equal marks.
• Each full question is for 20 marks.
• There will be two full questions (with a maximum of four sub questions) from each module.
• Each full question will have sub question covering all the topics under a module.
• The students will have to answer five full questions, selecting one full question from each module.

Textbook/ Textbooks

1 Principles of Fermentation Technology Stanbury& Whitaker , Second Edition, BH publications 1995

2 Biotechnology Text book of Industrial Microbiology W. Crueger and A. Crueger Sinauer Publishers 1990

Reference Books

1 Industrial Microbiology Casida Wiley 1986

2 Biotechnology : A Text Book of Industrial Microbiology T.D. Brock Smaeur Associates 1990

3 Comprehensive Biotechnology Moo-Young, M., Bull, A. T., Dalton, H. Pergamon Press 1987