MTech Bio-separation And Product Recovery syllabus for 2 Sem 2020 scheme 20BBC23

INTRODUCTION TO DOWNSTREAM PROCESSES

Role and importance of downstream processing in biotechnological processes. Problems and requirements of bio product purification. Economics of downstream processing in biotechnology; cost cutting strategies, characteristics of biological mixtures, process design criteria for various classes of by-products (high volume, low valve products and low volume, high valve products). Discussion of case studies.

PRIMARY SEPARATION AND RECOVERY PROCESS:

Cell disruption methods for intracellular products, removal of insoluble (particulate debris), centrifugation and filtration methods. Membrane based separations (dialysis, micro and ultra-filtration, reverse osmosis), theory design and configuration of membrane separation equipment application. Enrichment operations; precipitation methods (with salts, organic solvents and polymer extractive separations aqueous two phase extraction). Discussion of case studies.

ELECTROPHORETIC TECHNIQUES;

Theory of Electrophoresis; Classification; Applications : Moving boundary electrophoresis, Zone Electrophoresis, Gel Electrophoresis, Continuous Gel Electrophoresis, Disc gel Electrophoresis, Agarose Gel Electrophoresis, Cellulose Acetate, Starch Gel and page (Polyacrylamide gel electrophoresis) and SDS - Polyacrylamide, High voltage electrophoresis, Isoelectric focusing, Immunoelectrophoresis. Capillary electrophoresis. PFGE. Discussion of case studies.

INTRODUCTION TO MOLECULAR INERACTION AND CHROMATOGRAPHY:

Adsorption and absorption, Kinds of adsorption interactions. Adsorption characteristics, molecular orientation, adsorption isotherms: quantitative Relationships; adsorption from solutions, and the importance of Adsorption phenomena. Principle and classification of chromatography, important terms of chromatography, Partition chromatography – Single dimensional (Both Ascending and Descending) and 2-D chromatography; Paper chromatography, Thin layer chromatography, Adsorption Chromatography. Discussion of case studies.

ADVANCED PURIFICATION TECHNIQUES:

Ion Exchange Chromatography, Ge Filtration Chromatography, Affinity Chromatography. Principle of HPLC, theory and calculations, Instrumentation both analytical and preparative, Types of Columns, Detectors; Sampling Methods; Applications of HPLC, LCMS, GCMS. FPLC, HPTLC. Drying techniques, Crystallization, lyophilisation, Pervaporation, super liquid extraction, foam based separations, in situ product removal, Single step purification, Super critical extraction, online membrane separation, Discussion of case studies

Course outcomes:

• At the end of the course the student will be able to:
• Understand recovery and purification of biologically produced proteins and chemicals. Basic principles and engineering design of various separation processes including chromatography, electrophoresis, extraction, crystallization, and membrane separation.

Question paper pattern:

The SEE question paper will be set for 100 marks and the marks scored will be proportionately reduced to 60.

• The question paper will have ten full questions carrying equal marks.
• Each full question is for 20 marks.
• There will be two full questions (with a maximum of four sub questions) from each module.
• Each full question will have sub question covering all the topics under a module.
• The students will have to answer five full questions, selecting one full question from each module.

Textbook/ Textbooks

1 Bioseparation Engineering Endo, T.Nagamune, S. Katoh, T. Yonemoto Japan Nikko 1999

2 Handbook on Bioseparation (separation science and technology) Satinder Ahuja Academic Press 2000

Reference Books

1 Product Recovery in Bioprocess Technology BIOTOL ButterworthHeinemann, 1992

2 Downstream Processing and Bioseparation: Recovery and Purification of Biological Products Jean-François Hamel, Jean B. Hunter, American Chemical Society, 1990