MTech Bioprocess Engineering syllabus for 2 Sem 2022 scheme 22BBC21

HISTORY OF DEVELOPMENT OF FERMENTATION INDUSTRY:

The range of fermentation process, Microbial biomass, enzymes, metabolites, recombinant products, Transformation process, the component parts of Fermenter. Types of industrial bioprocesses; submerged, surface, solid state fermentations: aerobic, anaerobic and light based processes. The differences between laboratory, pilot, and manufacturing scale bioreactor experiments, Green biologics of fermentation technology, types of Reactor and reactor design, process economics. Discussions with case studies

SCREENING OF IMPORTANT METABOLITES FROM MICROBIAL SOURCES:

Primary and secondary screening of industrially important microbes, Screening methods, General Techniques in improvement of industrial strains, Isolation of auxotrophic mutants, resistant mutants, revertant mutants, Selection by induced mutants producing improved yields of secondary metabolites. Preservation and storage at reduced temperature; Agar slopes, liquid nitrogen, dehydrated form, dried culture and lyophilisation. Quality control of reservation of stock cultures

INTRODUCTION TO CULTURE MEDIUM AND FORMULATION:

Energy sources, Carbon & Nitrogen sources, Minerals, Growth factors, Buffers, Precursors and regulators, Oxygen and antifoam ingredients, Medium optimization. Substrates for solid state fermentation, Evaluation methods for complex Substrates differences based on product use.

STERILIZATION PROCESS AND INOCULUM DEVELOPMENT

Medium sterilization, Design for Batch sterilization process, Calculation of del factors and holding time. Design of continuous sterilization process, Sterilization of Fermenters, Feeds & liquid wastes, Filter sterilization of media. Discussions with case studies Development of Inoculum, criteria for transfer, development of inoculum in yeast, bacterial and mycelial processes, aseptic inoculation of plant fermenters. Inoculum development methods.

LABORATORY TO LARGE SCALE FERMENTATION PROCESSES:

Batch, Continuous culture, Synchronous, nonsynchronous growth kinetics, Feedback systems, comparison of Batch and Continuous culture in industrial processes and investigative tools. Fed batch culture, Applications of Fed back cultures Techniques and trends in Fermentation technology for the production of recombinant vaccines, therapeutic proteins, antibiotics and diagnostics. Discussions with case studies. Treatment and disposal procedure for industrial effluents.

Assessment Details (both CIE and SEE)

• The weightage of Continuous Internal Evaluation (CIE) is 50% and for Semester End Exam (SEE) is 50%.
• The minimum passing mark for the CIE is 50% of the maximum marks. Minimum passing marks in SEE is 40% of the maximum marks of SEE.
• A student shall be deemed to have satisfied the academic requirements and earned the credits allotted to each subject/ course if the student secures not less than 50% (50 marks out of 100) in the sum total of the CIE (Continuous Internal Evaluation) and SEE (Semester End Examination) taken together.

Continuous Internal Evaluation:

3. Three Unit Tests each of 20 Marks

4. Two assignments each of 20 Marks or one Skill Development Activity of 40 marks to attain the COs and POs

The sum of three tests, two assignments/skill Development Activities, will be scaled down to 50 marks CIE methods /question paper is designed to attain the different levels of Bloom’s taxonomy as per the outcome defined for the course.

Semester End Examination:

6. The SEE question paper will be set for 100 marks and the marks scored will be proportionately reduced to 50.

7. The question paper will have ten full questions carrying equal marks.

8. Each full question is for 20 marks. There will be two full questions (with a maximum of four sub-questions) from each module.

9. Each full question will have a sub-question covering all the topics under a module.

10. The students will have to answer five full questions, selecting one full question from each module .

Suggested Learning Resources:

Books

1. Biotechnology Text book of Industrial Microbiology, W. Crueger and A. Crueger, Sinauer Publishers, 1990

2. Downstream Process Technology: A New Horizon In Biotechnology, Nooralabettu Krishna Prasad, PHI, 2012

3. Industrial Microbiology, Casida, Wiley, 1986

4. Biotechnology : A Text Book of Industrial Microbiology, T.D. Brock, Smaeur Associates, 1990

5. Comprehensive Biotechnology, Moo-Young, M., Bull, A. T., Dalton, H. Pergamon Press, 1987

Course outcome (Course Skill Set)

At the end of the course the student will be able to :

CO1 Demonstrate strong basics in principles of fermentation technology L1, L2, L3,

CO2 Demonstrate strong basics numerical analysis L1, L2, L3,

CO3 Design and develop various fermentation processes L1, L2, L3,

Programm Outcomes

1 Engineering knowledge: Apply the knowledge of mathematics, science, engineering fundamentals, and an engineering specialization to the solution of complex engineering problems. PO1

2 Problem analysis: Identify, formulate, review research literature, and analyse complex engineering problems reaching substantiated conclusions using first principles of mathematics, natural sciences, and engineering sciences. PO2

3 Design/development of solutions: Design solutions for complex engineering problems and design system components or processes that meet the specified needs with appropriate consideration for the public health and safety, and the cultural, societal, and environmental considerations PO3