BRIEF REVIEW OF ELECTROMAGNETIC SPECTRUM AND ABSORPTION OF RADIATIONS:
Theory of spectroscopy, absorption of organic molecules, choice of solvent and solvent effects,modern instrumentation – design and working principle.Applications of UV-Visible spectroscopy (qualitative andquantitative analysis). Principles of vibrationalspectroscopy, frequency and factors influencingvibrational frequency, instrumentation and samplingtechniques, interpretation of spectra, applications inbiology. FT-IR-theory and applications, Attenuated TotalReflectance (ATR). Raman Spectroscopy, theory,instrumentation, and applications to biology. Discussionswith Case studies.
FUNDAMENTAL PRINCIPLES OF NMR:
Instrumentation, solvents, chemical shift, and factorsaffecting chemical shift, spin-spin coupling, couplingconstant, and factors influencing the value of couplingconstant, spin-spin decoupling, proton exchangereactions, FTNMR, 2D -NMR, NMDR, NOE, NOESY, COSY and applications in Pharmacy, interpretation ofspectra, C13 NMRIntroduction, Natural abundance, C13NMR Spectra and its structural applications. Discussionswith Case studies
BASIC PRINCIPLES AND INSTRUMENTATION OF ION FORMATION AND TYPES:
Fragmentationprocesses and fragmentation pattern, Chemical ionizationmass spectroscopy (CIMS), Field Ionization MassSpectrometry (FIMS), Fast Atom Bombardment MS(FAB MS), Matrix Assisted laser desorption / ionizationMS (MALDI-MS), GC-MS. LC-MS. MS-MS.Discussions with Case studies.
INTRODUCTION TO X-RAY:
Generation of X-rays, X-ray diffraction, Bragg’s law, X-ray powder diffraction, interpretation of diffraction patterns and applications. Single crystal diffractions of biolomolecules. Fibre diffraction. Neutron diffraction. XAFS. ORD Principle, Plain curves, curves with cotton effect, octant rule and its applications with example, circular dichroism and its relation to ORD. Discussions with Case studies.
CHROMATOGRAFIC TECHNIQUES:
Classification of chromatographic methods based on mechanism of separation: paper chromatography, thin layer chromatography, ion exchange chromatography, column chromatography and affinity chromatography – techniques and applications. Gas Chromatography: Theory and principle, column operation, instrumentation, derivatisation methods and applications. HPLC, LC-MS and applications in HPTLC. Discussions with Case studies