Laboratory Experiments:
1 Calculation minimum settling velocity of cells.
2 Calculation of fluidizing velocity of immobilized enzyme system
3 Rate of drying.
4 Extraction of antibiotics from different organic solvent andstudying its efficiency of extraction.
5 Study of adsorption of proteins on matrix by differentisotherms.
6 Study of mass transfer effect on reaction of immobilizedenzymes.
7 Isolation of genomic DNA from Bacteria/ Plant/ Animal cells and its quantification.
8 Study of Denaturation and Renaturation of DNA and calculation of Tm value of DNA.
9 Isolation of total RNA from E.coli.
10 Preparation of Competent E coli cells and Transformation.
11 Isolation of Plasmid DNA and its purification.
12 Restriction analysis and agarose electrophoresis of DNA
Course outcomes:
On the completion of this laboratory course, the students will be able to have hands on experience on,
• All laboratory experiments are to be includedfor practical examination.
• Strictly follow the instructions as printed on the coverpage of answerscript for breakup of marks.
• Change of experiment is allowed only once and Marks allotted to the Procedure part will be madezero.