INTRODUCTION:
Bioprocess development an interdisciplinary challenge, introduction to engineering calculations, presentation of analysis of data, regulatory constraints for bioprocess engineering. Bioprocess engineering and technology. Role of a Chemical engineer in a bioprocess industry. Classification of microorganisms, Taxonomy, Environmental and Industrial microbiology.
ENZYMES:
Introduction, definition and enzyme classification, enzyme kinetics, various models, Experimentally determining rate parameters for MM Kinetics, complex enzyme kinetics, effect of pH and temperatures, insoluble substrates,
IMMOBILISED ENZYME SYSTEMS:
methods and limitation of immobilization, Effects of diffusion and reaction on kinetics of immobilized enzymes, Effect of other environmental parameters like pH and temperature.
GROWTH KINETICS OF MICROORGANISMS:
Transient growth kinetics (Different phases of batch cultivation). Quantification of growth kinetics: Substrate limited growth, Models with growth inhibitors, Logistic equation, Filamentous cell growth model. Continuous culture: optimum dilution rate in an ideal Chemostat. Introduction to fed-batch reactors. Immobilized Cells: Formulations, Characterization and Applications
MIXED CULTURES:
Introduction to mixed cultures, Major Classes of Interactions: Simple Models, Competition between two species, Prey-Predator system, Lotka-Volterra Model Web Interaction, Population dynamics in models of mass action form.
Anaerobic process:
Ethanol, lactic acid, acetone-butanol production.
Aerobic Processes:
Citric Acid, Baker’s Yeast, Penicillin, High fructose corn syrup production.