18CH46 Instrumental Analysis syllabus for CH

CHROMATOGRAPHY:

Classification of chromatographic methods based on the mechanism of separation. Column Chromatography: Adsorption and partition, theory, preparation, procedure and methods of detection. Thin Layer Chromatography: Theory, preparation, procedures, detection of compounds. Paper Chromatography: Theory, different techniques employed, filter papers used, qualitative and quantitative detection .Counter – current extraction, solid phase extraction techniques, gel filtration.

GAS CHROMATOGRAPHY:

Introduction, fundamentals, instrumentation, columns: preparation and operation, detection, dramatization. . HPLC: Principles and instrumentation, solvents and columns, detection and applications, HPTLC: Theory and principle, instrumentation, elution techniques.

Introduction, electromagnetic spectrum, absorbance laws and limitations, instrumentation-design and working principle, chromophore concept, auxochromes, Wood-Fisher rules for calculating absorption maximum, applications of UV-Visible spectroscopy . IR spectroscopy: Basic principles-Molecular vibrations, vibrational frequency, factors influencing vibrational frequencies, sampling techniques, instrumentation, interpretation of spectra, FT-IR, theory and applications.

MASS SPECTROSCOPY:

Theory, ionization techniques: electron impact ionization, chemical ionization, field ionization, fast atom bombardment, plasma desorption, fragmentation process: types of fission, resolution, GC/MS, interpretation of spectra and applications for identification and structure determination.

NMR:

Theory, instrumentation, chemical shift, shielding and de-shielding effects, splitting of signals, spinspin coupling, proton exchange reactions, coupling constant (J), nuclear over Hauser effect (NOE), 13CNMR spectra and its applications, 2D-NMR, COSY and applications.

Course Outcomes:

On successful completion of this course students will be able to

• Discuss types of spectroscopy, instrumentation and applications of UV Spectroscopy
• Explain theory, instrumentation and applications of IR spectroscopy
• Explain theory, instrumentation and applications of NMR spectroscopy
• Discuss principle, instrumentation and applications of Mass Spectroscopy, Flame Emission Spectroscopy (FES) and Atomic Absorption Spectroscopy (AAS)
• Discuss principle, instrumentation and applications of polarography
• Discuss classification of chromatography and explain Thin Layer, Gas Chromatography and High Performance Liquid Chromatographic methods.

QUESTION PAPER PATTERN:

• The question paper will have ten questions. · Each full Question consisting of 20 marks
• There will be 2 full questions (with a maximum of four sub questions) from each module.
• Each full question will have sub questions covering all the topics under a module.
• The students will have to answer 5 full questions, selecting one full question from each module

TEXT BOOKS:

1. Instrumental Methods of Chemical Analysis by B.K Sharma

2. Organic Spectroscopy by Y.R Sharma.

REFERENCES:

1. Text book of Quantitative Chemical Analysis by Vogel’s A.I.

2. Organic Spectroscopy by William Kemp