An Introduction to Instrumental Methods:
Terms associated with Chemical analysis, Classification of instrumental techniques, A review of important consideration in analytical methods, Basic functions of instrumentation, Fundamental Laws of photometry (Text book 1).
IR Spectroscopy:
Basic Components of IR Spectrophotometers, monochromators- littrow mounting, Fourier Transform IR Spectroscopy (Text book 2).
UV and Visible Spectrometers –Instrumentation:
Radiation Sources, Wavelength selection: absorption filters, interference filters, Detector, Readout modules(Text book 1), Instruments for absorption photometry: single beam and double beam spectrophotometer. (Text book 2)
Flame Emission and Atomic Absorption Spectroscopy:
Introduction, Instrumentation for flame spectrometric methods, Flame emission spectrometry, atomic absorption spectrometry, Atomic fluorescence spectrometry, Interferences associated with Flames & furnaces, applications, comparison of FES and AAS. (Text book 1).
Gas Chromatography:
Chromatograph, Basics parts of a chromatograph: carrier gas supply, sample injection system, chromatographic columns: packed column & capillary column, Detectors: katharometer cell, differential flame ionization detector, electron capture detector.(Text book 2).
HPLC Instrumentation:
Mobile –phase delivery system sample introduction, separation of columns, Detectors– Ultraviolet Photometers & Spectrophotometers, electrochemical detector (amperometric detector), Differential refractometer. (Text book 1).
Blood analyzer:
Introduction, Blood pH measurements: electrodes for blood pH measurement, measurement of blood pCO2, pO2 , A Complete blood gas analyzer.
Air pollution monitoring instruments:
Carbon monoxide (CO) -Non-dispersive infrared analyzer, Sulphur dioxide (SO2)-Conductivitimetry, UV fluorescence method, Nitrogen oxides-Using CO laser, laser opto-acoustic spectroscopy, Hydrocarbons-Flame ionization detector, Ozone-Chemiluminescence, Automated wet chemical air analysis,
Water pollution monitoring instruments.
(Text book 2)
Course Outcomes:
1. The students get well versed with the principle, construction and working of various analytical instrumentation.
2. Students get detailed information about the application of analytical techniques in medicine, Industry, etc.
Question Paper Pattern:
• The question paper will have TEN questions.
• Each full question carry 20 marks
• There will be TWO full questions (with maximum of THREE sub questions) from each module.
• Each full question will have sub questions covering all the topics under a module.
• The students will have to answer FIVE full questions, selecting ONE full question from each module.
Textbooks:
1. Instrumental Methods of Analysis, 7th edition. H.H. Willard, L.L. Merritt, J.A. Dean, F.A. Settle, CBS Publishing & Distribution (Module 1, Module 2, Module 3, Module 4HPLC)
2. Handbook of Instruments – R.S. Khandpur, Tata McGraw Hill (Module 1-IR Spectroscopy, Module 4, Module 5)
Reference Books:
1. Braun R.D., Introduction to Instrumental Analysis, McGraw –Hill Singapore,2006.
2. Frank G. Kerry Industrial Gas Handbook: Gas Separation and Purification, Taylor and francis group, 2007
. 3. Principles of Instrumental Analysis 5th Edition – Douglas A. Skoog, F. James Holler, Timothy A. Niemen, Thomason Brooks/ Cole