General Introduction To Spectroscopy - DefineSpectroscopy, Types of spectroscopy, Absorption spectrum,Emission spectra, Wave length and Wave number,Electromagnetic radiation, Visible spectrum, Stokes’sshift, Hypochromicity, transmittance.Introduction, basic principles and instrumentation -Infrared Spectroscopy, Flame Photometry, AtomicAbsorption Spectroscopy and Mass Spectrometry
Radiochemical Techniques – Define radioactivity, half lifeof radioactive element, radioactive isotopes, Inducedradioactivity, GM Counter, Gas ionization detector,Scintillation counter, Quenching, Radiodating, Radioactivetracer, Autoradiography, Radioimmuno assay.Electrophoretic Methods – Principle, Types – free solutionmethod and zone electrophoresis, Electrophoretic mobility,Factors affecting electrophoretic mobility.
Principles of polarographic measurements,polarograms, Description and working of dropping mercuryelectrode. Current and concentrations relationship.Supporting electrolyte. Limiting current, half wavepotential. Factors affecting half wave potential. Migrationcurrent, Residual current and diffusion current. Modes ofoperation. Rapid scan polarography, differential pulsepolarography, sinusoidal a.c. polarography. Applications ofpolarography-Identification and determination ofconcentration of analyte.
Introduction to Chromatography: Classification - Theory- distribution coefficient, rate of travel, retention time,retention volume, adjusted retention volume, specificretention volume, column capacity, separation number, peakcapacity, shapes of chromatic peak, column efficiency,resolution, optimization of column performance,Chromatogram, Void volume.Thin Layer Chromatography: Stationary phase, mobilephase, sample application, development techniques –evaluation and documentation, advantages anddisadvantages of TLC.
Gas Chromatography: Principle, carrier gas, stationeryphase, instrumentation, sample injection, column detectors(TCD, FID, ECD), effect of temperature on retention,qualitative and quantitative analysis.High Performance Liquid Chromatography: Principle,instrumentation, column, sample injection, detectors(absorbance, refractive index, electrochemical), mobilephase selection, ion pair chromatography.